ThiC Is an [Fe-S] Cluster Protein That Requires AdoMet To Generate the 4-Amino-5-hydroxymethyl-2-methylpyrimidine Moiety in Thiamin Synthesis†
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چکیده
منابع مشابه
Histological investigations on the liver-disturbing action of 2-methyl-4-amino-5-hydroxy-methylpyrimidine.
Abderhalden (1) injected the pyrimidine and thiazole moieties of thiamine in mice and rats with the object of investigating the biosynthesis of thiamine and observed the induction of running fits. Its cause was further proved to be the pyrimidine moiety, i.e., 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP). Makino and Sasaki (2) reported the induction of running fits after in jecting this com...
متن کاملTomato LeTHIC is an Fe-requiring HMP-P synthase involved in thiamine synthesis and regulated by multiple factors.
Thiamine is a key primary metabolite which is necessary for the viability of all organisms. It is a dietary requirement for mammals because only prokaryotes, fungi and plants are thiamine prototrophs. In contrast to the well documented biosynthetic mechanism in bacteria, much remains to be deciphered in plants. In this work, a tomato thiamine-auxotrophic (thiamineless, tl) mutant was characteri...
متن کاملInhibition of Fe-S cluster biosynthesis decreases mitochondrial iron export: evidence that Yfh1p affects Fe-S cluster synthesis.
Decreased expression of Yfh1p in the budding yeast, Saccharomyces cerevisiae, and the orthologous human gene frataxin results in respiratory deficiency and mitochondrial iron accumulation. The absence of Yfh1p decreases mitochondrial iron export. We demonstrate that decreased expression of Nfs1p, the yeast cysteine desulfurase that plays a central role in Fe-S cluster synthesis, also results in...
متن کاملVitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC.
Vitamin B1 (thiamin) is an essential compound in all organisms acting as a cofactor in key metabolic reactions and has furthermore been implicated in responses to DNA damage and pathogen attack in plants. Despite the fact that it was discovered almost a century ago and deficiency is a widespread health problem, much remains to be deciphered about its biosynthesis. The vitamin is composed of a t...
متن کاملDesign, Synthesis and Anti-Tubercular Activity of Novel 1, 4-Dihydropyrine-3, 5-Dicarboxamide Containing 4(5)-Chloro-2-Ethyl-5(4)-Imidazolyl Moiety
Current researches have showed that N3, N5-diaryl-2, 6-dimethyl -1, 4-dihydropyrine-3, 5- dicarboxamide analogues demonstrate notable anti-tubercular activity. In this study, Hantzsch condensation was used to design and synthesize new analogues of dihydropyridine (DHP). Different diary carboxamides were inserted at positions 3 and 5 of the DHP ring. 4(5)-chloro-2-ethyl-5(4)-imidazolyl moiety wa...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2008
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi8010253